Bo Zheng, Chinese University of Hong Kong
January 21, 2011
11:00 AM - 12:00 PM
Bioanalysis in Poly(dimethyl siloxane)-Based Microchannels
Abstract:
Cross linked poly(dimethyl siloxane) (PDMS) is a transparent rubber and is the most often used material in fabricating microfluidic devices. A unique property of PDMS is the relatively large solubility of air in PDMS. Small molecules, including oxygen and nitrogen, diffuse in PDMS at a similar rate as they do in water. As a result, degassed PDMS can be used as a vacuum source with proper microfluidic design. Two applications in bioanalysis based on the degassed PDMS will be presented. First, a nanoliter liquid dispenser was developed and applied to protein crystallization. Second, PDMS microchannel-based viscometers for Newtonian fluid and for power law non-Newtonian fluid were developed using degassed PDMS to drive the flow of the fluid. In both applications, only nanoliter to microliter volume of the sample fluid was needed, and the analysis process was simple and robust.
Date posted
Jun 17, 2019
Date updated
Jun 17, 2019